Abstract The present study aimed to minimize the pollution effects of traditional insecticides on the environment by development and synthesis of bioinsecticide and to evaluate some insect growth regulators and plant extract as insecticidal agents. Stock colony of Schistocerca gregaria was used in this study which were kept in cages and fed on leaves of Castor plant. Treatment the fifth nymphal instar of locusts by using insect growth regulator (Match), rice plant extract and Triton-X100 combined with rice plant extract were carried out. Results showed that, the treatment with different concentrations of Match (50, 75 and 100 ppm) resulted in nymphal mortality of 0.0, 12.33 and 21.01% respectively and percentages of adult malformed with 89.99, 73.34 and 72.33% respectively comparing with control. Treatment with different concentrations of Oriza sativa bran extract (500, 700 and 1000 ppm) caused nymphal mortality (13.43, 16.85 and 20.68%) and failure to ecdysis to adult (35.66, 57.15 and 79.32%) with the increase of concentrations. The biological activities resulting from adding Triton-X100 (0.01%) to different concentrations of the extract of rice bran showed considerable increase in nymphal mortality (23.33 , 28.66 and 40.66%) whereas, the percentages of adult malformed were decreased by 29.66 , 53.33 and 59.33%. The current study showed that, Match and rice bran extract caused malfunction and decomposition of the components in the muscles of the desert locust. Also, the pattern of acid phosphatase, which recorded a lower diversity in each of control and treated insects which may be due to the lower metabolic rate in treated samples.